Signal Transduction: ECM Bioscience Antibody Sampler Kits
Catalog
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Antibody Sampler Kit Description
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AK6060
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Actin & Tubulin Antibody Sampler Kit
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CK6120
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b-Catenin Phospho-Regulation Antibody Sampler Kit
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CK6150
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g-Catenin Phospho-Regulation Antibody Sampler Kit
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CK6040
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Cofilin Phospho-Regulation Antibody Sampler Kit
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CK6180
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Coronin-1B Phospho-Regulation Antibody Sampler Kit
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CK6200
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CRMP2 Phospho-Regulation Antibody Sampler Kit
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EK6160
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EGFR Phospho-Regulation Antibody Sampler Kit
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MK6050
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MAP Kinase Activation Antibody Sampler Kit
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MK6170
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Muscle Atrophy Ubiquitin Ligase Antibody Sampler Kit
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PK6140
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p38 MAP Kinase Phospho-Regulation Antibody Sampler Kit
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PK6070
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Paxillin Phospho-Regulation Antibody Sampler Kit
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RK6080
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Raf Phospho-Regulation Antibody Sampler Kit
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RK6100
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Robo1 & Robo2 Antibody Sampler Kit
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SK6010
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Sphingosine Kinase Phospho-Regulation Antibody Sampler Kit
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SK6030
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Stat Phospho-Regulation Antibody Sampler Kit
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VK6020
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Vav Phospho-Regulation Antibody Sampler Kit
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VK6090
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VEGFR Phospho-Regulation Antibody Sampler Kit
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WK6110
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N-WASP Phospho-Regulation Antibody Sampler Kit
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WK6130
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WAVE Phospho-Regulation Antibody Sampler Kit
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VK6090
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VEGFR Phospho-Regulation Antibody Sampler Kit
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Protein Transfection Reagents from Abbiotec
Lipodin™ Lipid-Based Reagents
Lipodin™ transports biologically active peptides, proteins and antibodies directly into living cells. Lipodin™ represents an alternative to nucleic acid transfection for functional studies. Within few hours, protein activity can be observed in living or fixed cells. Regardless of serum addition, Lipodin™ is ideal for monitoring kinetics of biological activities in established cell lines and primary cells, including neurons
Features
· Study living cells, no fixation needed
· Deliver in many cell lines and primary cells including neurons
· Up to 95% efficiency in less than 5 hours
· Serum compatible, non cytotoxic
(A) Human IgG labeled with FITC (1 ìg) was delivered in monkey kidney fibroblast COS-7 cells and fluorescence observed 4 h posttransfection.
(B) An antibody conjugated to AlexaFluor 488 (0.5 ìg) was delivered in primary neurons and glial cells. Fluorescence
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was observed 24 h post-transfection.
(C) An anti-Nuclear Pore Complex antibody conjugated to AlexaFluor®488 (0.5 ìg) was delivered in human non-small cell lung carcinoma A549 cells and the nuclear envelope localization (white arrow) observed 6 h post-transfection.
(D) An anti-Nuclear Pore Complex antibody (0.5 ìg) was delivered in hamster ovary epithelial CHO cells and apoptotic effect observed 72 h post-transfection. Control cells transfected with Lipodin-Ab alone are shown in inset.
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Product
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Unit
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Cat. No.
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Lipodin-Pro™ for protein transfection
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25 rxn
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500100
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100 rxn
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500110
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Lipodin-Ab™ for antibody delivery
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25 rxn
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500115
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100 rxn
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500120
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