Format: Purified - Reactivity: human - rat - Xenopus
Articlecode: 03-61244
Product: Anti-20S Proteasome (multicatalytic proteinase/ 20S cylinder particle)
Amount: 50 microliter
CATEGORY: Mouse monoclonal
CLONE DETERMINATION: P27 (26S-104)
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Nuclear 26S complexes of Xenopus laevis oocytes
FORM: Ascites
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Xenopus laevis; XLKE-A6 (X. laevis); PLC (human); RVF (rat); Ptk2 (rat and kangaroo)
INCUBATION TIME: 20-30 min. at room temperature longer incubation may result in loss of soluble antigen
SPECIAL NOTE: Recognizes the 20S subcomplex within the 26S hetero-oligomeric Protein complex and the free cytosolic form of 20S cylinder particles.The epitope is resistant to formaldehyde fixation (up to 4%)
APPLICATION: Preferably for Immunofluorescence microscopy
Immunoblotting (Western)
Immunoprecipitation
Affinity Chromatography
POLYPEPTIDE REACTING: p27 subunit of 20S cylinder particles
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
Peters, J.M. et al. (1994) J. Biol. Chem. 269: 7708-7718
Peters, J.M.: Proteasomes: protein degradation machines of the cell. TIBS, 19, September 1994.
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