Format: Supernatant - Reactivity: human - rat - Xenopus
Articlecode: 03-65144
Product: Anti-26S Proteasome (multicatalytic proteinase/ 20S cylinder particle)
Amount: 1 ml
CATEGORY: Mouse monoclonal
CLONE DETERMINATION: P27 (26S-104)
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Nuclear 26S complexes of Xenopus laevis oocytes
FORM: Hybridoma culture supernatant
INCUBATION TIME: 20-30 min. at room temperature, longer incubation may result in loss of soluble antigen
SPECIAL NOTE: Recognizes the 20S subcomplex within the 26S heterooligomeric protein complex and the free cytosolic form of 20S cylinder particles. The epitope is resistant to formaldehyde fixation (up to 4%)
APPLICATION: Preferably for Immunofluorescence microscopy
Immunoblotting (Western)
Immunohistochemistry
POLYPEPTIDE REACTING: p27 subunit of 20S cylinder particles
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Xenopus laevis; XLKE-A6 (X. laevis); PLC (human); RVF (rat); Ptk2 (rat and kangaroo)
WORKING DILUTION: Ready to use for immunofluorescence
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
Peters, J.M. et al. (1994) J. Biol. Chem. 269: 7708-7718
Peters, J.M.: Proteasomes: protein degradation machines of the cell. TIBS, 19, September 1994
|
