Format: Supernatant; Reactivity: human, rat, dog
Articlecode: 03-651102
Product: Anti-Adipophilin
Catalog #: 03-651102
Amount: 1 ml
CATEGORY: Mouse monoclonal
CLONE DETERMINATION: AP 125
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Synthetic peptide (aa 5-27 from N-terminus of human adipophilin)
FORM: Cell culture supernatant
ANTIGEN RECOGNIZED IN SPECIES: human, rat, dog; negative with bovine, mouse
SPECIFICITY: Specific for adipophilin present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir*
APPLICATION: - Immunoblotting
- Immunohistochemistry of frozen tissue, paraffin embedded tissue after microwave treatment.
POLYPEPTIDE REACTING: Adipophilin, Mr 48,100.13 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pl 6.72
REACTIVITIES ON
CULTURED CELL LINES: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK; negative with glioma.
WORKING DILUTION: Ready to use for immunohistochemistry
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
Heid, H.W.et al. (1997) (submitted for publication)
Heid, HWet al. (1996) Biochem J. 320: 1025-1030
*Steiner, S. et al. (1996). Biochem. Biophys. Res. 218: 777-778
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