Hepatitis B surface antigen is bound on the surface of the
microtiter strips. Anti-HBs in the sample binds to HRP-labelled
HBsAg in conjugate and to HBsAg coated in the plate. The
unbound constituents are removed by washing and the
enzymatic substrate is added to the plate and reacts with
enzyme. The reaction is terminated by addition of stopping
solution and developed colour is analyzed at 450 nm with
spectrophotometer.
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