Format: PE; Reactivity: mouse
Articlecode: 12-9972-83
Contents: Phycoerythrin (PE) anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2)
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 122
Isotype: Rat IgG2a, ê
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Staining of in vitro BM-derived dendritic cells from BALB/c with 0.125 ìg PE Rat IgG2a Iso Cntrl (cat. 12-4321) (open histogram) or 0.06 ìg PE anti-mouse B7-DC (122)(colored histogram). Total viable cells were used for analysis. |
Available Formats of This Product
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Format
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Reported Applications
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14-9972
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N/A
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N/A
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13-9972
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N/A
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N/A
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11-9972
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12-9972
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Description
The 122 monoclonal antibody reacts with mouse B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages in the mouse. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. 122 has been demonstrated to block binding of TY25 (Cat. No. 14-5986), another mAb specific for mouse B7-DC.
Usage
For research use only, not for diagnostic or therapeutic use. 122 has been reported for use in flow cytometric analysis.
Applications Tested
The 122 antibody has been tested by flow cytometric analysis of mouse dendritic cell suspensions and mouse B7-DC transfected cells. This can be used at less than or equal to 0.125 ìg per million cells in a 100 ìl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 12-4321 PE Rat IgG2a Isotype Control
Cat. 12-5986 PE anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 13-5986 Biotin anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 14-5986 Affinity Purified anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 16-5986 Functional Grade Purified anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 11-9972 Fluorescein (FITC) anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2) (clone 122)
Cat. 13-9972 Biotin anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2) (clone 122)
Cat. 14-9972 Affinity Purified anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2) (clone 122)
References
Ishida M, Iwai Y, Tanaka Y, Okazaki T, Freeman GJ, Minato N, Honjo T. 2002. Differential expression of PD-L1 and PD-L2, ligands for an inhibitory receptor PD-1, in the cells of lymphohematopoietic tissues. Immunol Lett. 84(1):57-62. Carter L, Fouser LA, Jussif J, Fitz L, Deng B, Wood CR, Collins M, Honjo T, Freeman GJ, Carreno BM. 2002. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 32(3):634-43.
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