Format: PE; Reactivity: mouse
Articlecode: 12-5986-81
Contents: PE anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: TY25
Isotype: Rat IgG2a, ê
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Staining of non-transfected (left) and mouse B7-DC transfected (right) cells with 0.25 µg PE Rat IgG2a iso cntrl (cat.12-4321) (open histogram) or 0.06 µg PE anti-mouse B7-DC (TY25)(colored histogram). Total viable cells were used for analysis. |
Available Formats of This Product
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Cat. No.
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Format
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Excite
(nm)
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Emit
(nm)
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Reported Applications
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12-5986
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13-5986
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N/A
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N/A
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14-5986
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N/A
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N/A
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16-5986
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N/A
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N/A
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg
(unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
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Description
The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2. B7-DC, a member of the B7 family, has a predicted molecular weight of ~25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by sub-populations of dendritic cells, monocytes and macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, but binds PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. TY25 is a useful tool to study the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in mouse.
Usage
For research use only, not for diagnostic or therapeutic use. The TY25 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The TY25 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and B7-DC transfected cells. This can be used at less than or equal to 0.5 ìg per million cells in a 100 ìl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 12-4321 PE Rat IgG2a Isotype Control
Cat. 13-5986 Biotin anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 14-5986 Affinity Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 16-5986 Functional Grade Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 11-9972 Fluorescein (FITC) anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone 122)
Cat. 12-9972 Phycoerythrin (PE) anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone 122)
Cat. 13-9972 Biotin anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone 122)
Cat. 14-9972 Affinity Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone 122)
References
Aramaki O, Shirasugi N, Takayama T, Shimazu M, Kitajima M, Ikeda Y, Azuma M, Okumura K, Yagita H, Niimi M. 2004. Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation. Jan 15;77(1):6-12.
Tseng SY, Otsuji M, et al. 2001. B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells. J Exp Med 193(7): 839-46.
Carter L, Fouser L, et al. 2002. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol 32(3): 634-43.
Latchman Y, Wood CR, et al. 2001. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol 2(3): 261-8.
Yamazaki T, Akiba H, et al. Expression of programmed death 1 ligands by murine T cells and APC. J Immunol. 2002 Nov 15;169(10):5538-45.
Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. 2003. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 171(8):4156-63.
Mohammed Javeed I. Ansari, Alan D. Salama, Tanuja Chitnis, R. Neal Smith, Hideo Yagita, Hisaya Akiba, Tomohide Yamazaki, Miyuki Azuma, Hideyuki Iwai, Samia J. Khoury, Hugh Auchincloss, Jr. and Mohamed H. Sayegh. 2003. The Programmed Death-1 (PD-1) Pathway Regulates Autoimmune Diabetes in Nonobese Diabetic (NOD) Mice. J Exp Med 198 (1): 63-69.
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