Format: Purified; Reactivity: human
Articlecode: 14-5888-80
Contents: Affinity Purified anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MIH18
Isotype: Mouse IgG1, ê
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Staining of human in vitro derived dendritic cells with 0.25 µg of purified mouse IgG1 isotype control (cat.14-4714) (open histogram) or 0.25 µg of purified MIH18 followed by biotin anti-mouse IgG (cat.13-4013) and SAv-PE (cat.12-4317) (colored histogram). Total viable cells were used for analysis. |
Available Formats of This Product
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Cat. No.
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Format
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Excite
(nm)
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Emit
(nm)
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Reported Applications
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14-5888
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N/A
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N/A
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16-5888
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N/A
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N/A
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13-5888
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N/A
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N/A
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12-5888
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg
(unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
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Description
The MIH18 monoclonal antibody reacts with human B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and belongs to the Ig superfamily. B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. MIH18 is a useful tool for studies to elucidate the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in humans. MIH18 is a blocking antibody.
Usage
For research use only, not for diagnostic or therapeutic use. The MIH18 antibody has been reported for use in flow cytometric analysis. Applicability of this mAb for other assays, including immunohistochemical staining, immunoprecipitation and functional studies, is currently under investigation. (Please use Functional Grade purified MIH18, cat. 16-5888, in functional assays.)
Applications Tested
The MIH18 antibody has been tested by flow cytometric analysis of mature dendritic cells and human peripheral blood leukocytes. This can be used at less than or equal to 0.5 ìg per 100 ìl blood (or per 1 million cells in 100 ìl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 11-4011 FITC Anti-Mouse IgG
Cat. 13-4013 Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317 Streptavidin-PE (Phycoerythrin)
Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714 Affinity Purified Mouse IgG1, K Isotype Control
Cat. 12-5888 PE anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone MIH18)
Cat. 13-5888 Biotin anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone MIH18)
Cat. 16-5888 Functional Grade Purified anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone MIH18)
References
Youngnak-Piboonratanakit P, Tsushima F, Otsuki N, Igarashi H, Machida U, Iwai H, Takahashi Y, Omura K, Yokozeki H, Azuma M. 2004. The expression of B7-H1 on keratinocytes in chronic inflammatory mucocutaneous disease and its regulatory role. Immunol Lett. 15;94(3):215-22.
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