Format: PE; Reactivity: mouse; B and T lymphocyte attenuator
Articlecode: 12-5950-82
Contents: Phycoerythrin (PE) anti-mouse BTLA (CD272, B and T lymphocyte attenuator)
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 6F7
Isotype: Mouse IgG1, ê
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Staining of BALB/c splenocytes with APC anti-mouse/human CD45R(B220)(RA3-6B2) (cat. 17-0452) and 0.125 ìg PE Mouse IgG1, Iso Cntrl (cat. 12-4714) (left) or 0.06 ìg PE anti-mouse BTLA (6F7) (right). Total viable cells were used for analysis. |
Available Formats of This Product
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Cat. No.
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Format
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Excite
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Emit
(nm)
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Reported Applications
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14-5950
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N/A
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N/A
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16-5950
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N/A
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N/A
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13-5950
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N/A
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N/A
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12-5950
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg
(unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
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Description
The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in the thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA has been implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.
Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes . It is not understood why there is a discrepancy in thymocyte staining however clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7 in splenocytes and bone marrow cells.
Usage
For research use only, not for diagnostic or therapeutic use. This 6F7 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 6F7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 ìg per million cells in a 100 ìl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 13-5950 Biotin anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking (clone 6F7)
Cat. 14-5950 Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking (clone 6F7)
Cat. 16-5950 Functional Grade Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking (clone 6F7)
Cat. 14-5953 Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) (clone 3F9.D12)
Cat. 14-5954 Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) (clone 6H6 (BTLA))
Cat. 14-5955 Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) (clone 6G3)
Cat. 12-5956 Phycoerythrin (PE) anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Non-Blocking (clone 8F4)
Cat. 13-5956 Biotin anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Non-Blocking (clone 8F4)
Cat. 14-5956 Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Non-Blocking (clone 8F4)
References
Watanabe N, Gavrieli M, Sedy JR, Yang J, Fallarino F, Loftin SK, Hurchla MA, Zimmerman N, Sim J, Zang X, Murphy TL, Russell JH, Allison JP, Murphy KM. 2003. BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1. Nat Immunol. 4 (7): 670-9.
Sedy JR, Gavrieli M, Potter KG, Hurchla MA, Lindsley RC, Hildner K, Scheu S, Pfeffer K, Ware CF, Murphy TL, Murphy KM. 2005. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. (6)1: 90-8.
Hurchla MA, Sedy JR, Gavrielli M, Drake CG, Murphy TL, Murphy KM. 2005. B and T Lymphocyte Attenuator exhibits structural expression polymorphisms and is highly induced in CD4+ T cells. The Journal of Immunology. 174: 3377-3385.
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