Contents: Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) Sizes: 25 ug, 100 ug Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: 6G3 Isotype: Mouse IgG1, ê

Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and .125 ìg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (left) or .125 ìg of Purified anti-mouse BTLA (6G3) (right) followed by Biotin Anti-Mouse IgG (cat. 13-4013) and Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5955	Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator)	N/A	N/A	FC

The 3F9.D12 antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.

Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes . It is not understood why there is a discrepancy in thymocyte staining however clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7 in splenocytes and bone marrow cells.

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control
Cat. 12-5950    Phycoerythrin (PE) anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking (clone 6F7)
Cat. 13-5950    Biotin anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking (clone 6F7)
Cat. 14-5950    Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking (clone 6F7)
Cat. 16-5950    Functional Grade Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking (clone 6F7)
Cat. 14-5953    Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) (clone 3F9.D12)
Cat. 14-5954    Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) (clone 6H6 (BTLA))
Cat. 12-5956    Phycoerythrin (PE) anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Non-Blocking (clone 8F4)
Cat. 13-5956    Biotin anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Non-Blocking (clone 8F4)
Cat. 14-5956    Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Non-Blocking (clone 8F4)

Watanabe N, Gavrieli M, Sedy JR, Yang J, Fallarino F, Loftin SK, Hurchla MA, Zimmerman N, Sim J, Zang X, Murphy TL, Russell JH, Allison JP, Murphy KM. 2003. BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1. Nat Immunol. 4 (7): 670-9.
Sedy JR, Gavrieli M, Potter KG, Hurchla MA, Lindsley RC, Hildner K, Scheu S, Pfeffer K, Ware CF, Murphy TL, Murphy KM. 2005. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. (6)1: 90-8.
Hurchla MA, Sedy JR, Gavrielli M, Drake CG, Murphy TL, Murphy KM. 2005. B and T Lymphocyte Attenuator exhibits structural expression polymorphisms and is highly induced in CD4+ T cells. The Journal of Immunology. 174: 3377-3385.