Format: Biotin; Reactivity: mouse; PDL-1, PDL1
Articlecode: 13-9971-81
Contents: Biotin anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 1-111A
Isotype: Rat IgG2a, ê
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Staining of C57Bl/6 splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and 0.06 ìg Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (left) or 0.06 ìg Biotin anti-mouse B7-H1 (1-111A)(right) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis. |
Available Formats of This Product
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Reported Applications
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14-9971
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N/A
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13-9971
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Description
The 1-111A monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. Preliminary testing suggests that 1-111A and MIH5 (Cat. No. 14-5982) cross-block each other.
Usage
For research use only, not for diagnostic or therapeutic use. The 1-111A antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 1-111A antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 ìg per million cells in a 100 ìl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317 Streptavidin-PE (Phycoerythrin)
Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321 Biotin Rat IgG2a Isotype Control
Cat. 12-5982 PE anti-mouse B7-H1 (CD274, B7H1, PDL-1, PD-L1) (clone MIH5)
Cat. 13-5982 Biotin anti-mouse B7-H1 (CD274, B7H1, PDL-1, PD-L1) (clone MIH5)
Cat. 14-5982 Affinity Purified anti-mouse B7-H1 (CD274, B7H1, PDL-1, PD-L1) (clone MIH5)
Cat. 16-5982 Functional Grade Purified anti-mouse B7-H1 (CD274, B7H1, PDL-1, PD-L1) (clone MIH5)
Cat. 12-5984 PE anti-mouse B7-H1 (B7H1, PD-L1, PDL-1, PDL1) (clone MIH6)
Cat. 13-5984 Biotin anti-mouse B7-H1 (B7H1, PD-L1, PDL-1, PDL1) (clone MIH6)
Cat. 14-5984 Affinity Purified anti-mouse B7-H1 (B7H1, PD-L1, PDL-1, PDL1) (clone MIH6)
Cat. 14-9971 Affinity Purified anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1) (clone 1-111A)
References
Ishida M, Iwai Y, Tanaka Y, Okazaki T, Freeman GJ, Minato N, Honjo T. 2002. Differential expression of PD-L1 and PD-L2, ligands for an inhibitory receptor PD-1, in the cells of lymphohematopoietic tissues. Immunol Lett. 84(1):57-62. Carter L, Fouser LA, Jussif J, Fitz L, Deng B, Wood CR, Collins M, Honjo T, Freeman GJ, Carreno BM. 2002. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 32(3):634-43.
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