Format: Purified; Reactivity: mouse; B7-RP1, B7RP1, ICOS-L, ICOSL, B7-H2
Articlecode: 14-5985-81
Contents: Affinity Purified anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HK5.3
Isotype: Rat IgG2a, ê
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Two-color surface staining of mouse splenocytes with anti-mouse B7RP-1 (HK5.3) PE and anti-mouse CD3e (145-2C11) FITC. Total viable cells were used for analysis. |
Available Formats of This Product
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Cat. No.
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Format
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Excite
(nm)
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Emit
(nm)
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Reported Applications
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12-5985
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13-5985
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N/A
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N/A
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14-5985
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N/A
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N/A
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16-5985
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N/A
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N/A
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg
(unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
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Description
The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ~40 kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.
Usage
For research use only, not for diagnostic or therapeutic use. The HK5.3 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking in functional assays. HK5.3 is not recommended for use in immunohistochemical staining. Preliminary immunoblotting (WB) using mouse spleen cells indicated that HK5.3 may not be effective for detection of mouse B7RP-1 by this application. Applicability of HK5.3 in immunoprecipitation has not been determined. (Please use Functional Grade purified HK5.3, cat. 16-5985, in functional assays.)
Applications Tested
The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ìg per million cells in a 100 ìl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317 Streptavidin-PE (Phycoerythrin)
Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321 Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811 FITC Anti-Rat IgG
Cat. 13-4813 Biotin Anti-Rat IgG (clone Polyclonal)
Cat. 12-5985 PE anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2) (clone HK5.3)
Cat. 13-5985 Biotin anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2) (clone HK5.3)
Cat. 16-5985 Functional Grade Purified anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2) (clone HK5.3)
References
Iwai H, Kozono Y, et al. 2002. Amelioration of collagen-induced arthritis by blockade of inducible costimulator-b7 homologous protein costimulation. J Immunol. 169(8): 4332-9. Carreno BM, Collins M. 2002. THE B7 FAMILY OF LIGANDS AND ITS RECEPTORS: New Pathways for Costimulation and Inhibition of Immune Responses. Annu Rev Immunol. 20: 29-53. Liu X, Bai XF, et al. 2001. B7H costimulates clonal expansion of, and cognate destruction of tumor cells by, CD8(+) T lymphocytes in vivo. J Exp Med. 194(9): 1339-48.
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