Format: Purified; Reactivity: human; CD275, B7-RP1, B7RP1, ICOSL, ICOS-L, B7-H2
Articlecode: 14-5889-80
Contents: Affinity Purified anti-human B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MIH12
Isotype: Mouse IgG1, ê
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Staining of normal human peripheral blood cells with Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or Purified anti-human B7RP-1 (MIH12) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis. |
Available Formats of This Product
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Cat. No.
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Format
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Excite
(nm)
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Emit
(nm)
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Reported Applications
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14-5889
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N/A
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N/A
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16-5889
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N/A
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N/A
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13-5889
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N/A
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N/A
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12-5889
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg
(unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
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Description
The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.
Reported Applications
For research use only, not for diagnostic or therapeutic use. This MIH12 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applicability of MIH12 for immunoprecipitation and immunoblotting (WB) has not been determined. (Please use Functional Grade purified MIH12, cat. 16-5889, in functional assays.)
Applications Tested
The MIH12 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and IFN-ã-activated PBMC. This can be used at less than or equal to 1 ìg per million cells in a 100 ìl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Youngnak-Piboonratanakit, P., F. Tsushima, et al. 2006. Expression and Regulation of Human CD275 on Endothelial Cells in Healthy and Inflamed Mucosal Tissues. Scand J Immunol 63: 191-198. (MIH12, FC, FA, IH/F, PubMed)
Carreno, B. M. and M. Collins 2002. THE B7 FAMILY OF LIGANDS AND ITS RECEPTORS: New Pathways for Costimulation and Inhibition of Immune Responses. Annu Rev Immunol 20: 29-53.
Liu, X., X. F. Bai, et al. 2001. B7H costimulates clonal expansion of, and cognate destruction of tumor cells by, CD8(+) T lymphocytes in vivo. J Exp Med 194(9): 1339-48.
Iwai, H., Y. Kozono, et al. 2002. Amelioration of collagen-induced arthritis by blockade of inducible costimulator-b7 homologous protein costimulation. J Immunol 169(8): 4332-9.
Related Products
Cat. 11-4011 FITC Anti-Mouse IgG
Cat. 13-4013 Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317 Streptavidin-PE (Phycoerythrin)
Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714 Affinity Purified Mouse IgG1, K Isotype Control
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