Format: FITC; Reactivity: mouse
Articlecode: 11-0043-85
Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD4 (L3T4)
Catalog Number: 11-0043
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RM4-4
Isotype: Rat IgG2b, ê
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Staining of C57Bl/6 splenocytes with .125 ìg of FITC Rat IgG2b Isotype Control (cat. 11-4031) (open histogram) or .06 ìg of FITC anti-mouse CD4 (RM4-4) (colored histogram). Cells in the lymphocyte gate were used for analysis. |
Available Formats of This Product
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Cat. No.
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Format
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Excite
(nm)
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Emit
(nm)
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Reported Applications
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14-0043
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N/A
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N/A
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16-0043
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N/A
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N/A
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13-0043
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N/A
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N/A
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11-0043
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12-0043
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg
(unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
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Description
The RM4-4 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by the majority of thymocytes, a subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with the protein tyrosine kinase lck through its cytoplasmic tail. Binding of RM4-4 does not block binding of the CD4 monoclonal antibodies RM4-5 or GK1.5.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This RM4-4 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This RM4-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 ìg per million cells in a 100 ìl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Fredrickson, G.G., and R.S. Basch. 1989. L3T4 antigen expression by hemopoietic precursor cells. J. Exp. Med. 169: 1473 - 1478.
Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus. Nature 349: 71 - 74.
Wu, L., M. Antica, G.R. Johnson, R. Scollay, and K. Shortman. 1991. Developmental potential of the earliest precursor cells from the adult mouse thymus. J. Exp. Med. 174: 1617 - 1627.
Godfrey, D.I., J. Kennedy, P. Mombaerts, S. Tonegawa, and A. Zlotnik. 1994. Onset of TCR-gene rearrangement and role of TCR-expression during CD3-CD4-CD8- thymocyte differentiation. J. Immunol. 152: 4783 - 4792.
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Cat. 11-4031 Fluorescein isothiocyanate (FITC) Rat IgG2b Isotype Control
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