human
Articlecode: MCYCM
Cytokine Control
Multi Control (bi-level) for: IL-1b, sIL-2R, IL-4, IL-6, IL-8, IL-10, TNF-a
- for in vitro use only -
Cat.-No.: MCYCM
Quantity: 2 x 2 mL
Technical Data
Required sample size: depending on the assay (25 - 100 µL)
Storage: refrigerated at 2 - 8 °C
Package size: depending on assay (80 - 20 tests)
Materials supplied, Storage and Stability
Components
|
Cat. No.
|
Content
|
Preparation
|
Storage at
|
Shelf life
|
Mix of recombinant IL-1b, sIL-2R, IL-4, IL-6, IL-8, IL-10, TNF-a;
in serum/buffer matrix
two different levels
(low, high)
|
MCYC1
MCYC2
|
1 Set
2 vials à 2 mL
|
lyophilized; reconstitute with 2 mL dist. H2O;
30 min. before use
|
30 min. before use - 20 °C in aliquots (freezing immediately after reconstitution)
|
4 months after reconstitution or until the
expiration date
|
Application
According the respective package insert of the different Milenia ?cytokine assays the both controls (low / high) are used in duplicates for internal quality control.
Target Values
The values provided her are intended as guidelines only. Because of many sources of interlaboratory
variations each laboratory should develop its own expected values.
Parameter
|
Level
|
Mean
|
Tolerance
|
Range
|
IL-1b (pg/mL)
|
MCYC1
|
162
|
21
|
120 - 203
|
|
MCYC2
|
502
|
67
|
368 - 636
|
sIL-2R (U/mL)
|
MCYC1
|
603
|
88
|
427 - 779
|
|
MCYC2
|
2,685
|
312
|
2,061 - 3,309
|
IL-4 (pg/mL)
|
MCYC1
|
58
|
8.2
|
33 - 83
|
|
MCYC2
|
202
|
13.3
|
162 - 242
|
IL-6 (pg/mL)
|
MCYC1
|
38
|
3.5
|
31 - 45
|
|
MCYC2
|
137
|
9
|
119 - 155
|
IL-8 (pg/mL)
|
MCYC1
|
92
|
6.3
|
73 - 111
|
|
MCYC2
|
227
|
11.1
|
194 - 260
|
IL-8 (pg/mL)
|
MCYC1
|
92
|
6.3
|
73 - 111
|
|
MCYC2
|
227
|
11.1
|
194 - 260
|
IL-10 (pg/mL)
|
MCYC1
|
74.3
|
14.1
|
46 - 102
|
|
MCYC2
|
228
|
41.1
|
145 - 310
|
TNF-a (pg/mL)
|
MCYC1
|
83
|
8.8
|
56 - 109
|
|
MCYC2
|
218
|
12.1
|
|
|
