EzWay DAB Substrate Kit
SPECIFICATION
		Diaminobenzidine (DAB) is the commonly used substrate for detection of peroxidase. 2 component system which develops a brown precipitate when reacted with horseradish peroxidase (HRP) in western blotting.
PROTOCOL
Add 1 volume of 5X DAB Solution to 4 volumes of PBS and add 30ul Substrate Solution (30% H2O2) per 50 ml 1x DAB solution prior to use. Incubate the membrane in the color reaction mixture for several minutes at room temperature or at 37°C After sufficient color development, wash the membrane with DW in order to stop the color reaction.
KIT CONTENTS
Items			Quantity
DAB Solution in PBS (5x)			500 ml x 2
Substrate Solution (30% H2O2)			1 ml
ORDERING INFORMATION
Cat. No.	Product			Size
K0335040	EzWay™ DAB Substrate Kit			10 mini-blots