EzWay DAB Western Blot Kit
|
|
SPECIFICATION
|
- Specific and accurate colorimetric protein detection using DAB
- Optimized all reagents supplied for quick and convenient western blot assay
- Affinity purified secondary antibodies included for versatility (rabbit & mouse)
- High sensitivity and clear background
- Easy to use
|
PROTOCOL
|
Transfer >> Rinsing >> Blocking >> Washing >> Reacting with primary antibody >> Washing >>
Reacting with secondary antibody >> Washing >> Color reaction
|
KIT CONTENTS
|
Items
|
Quantity
|
25X Transfer Buffer (Tris-Glycine)**
|
100 ml x 2
|
6X Blocking Solution (3% Casein/PBS)
|
100 ml
|
PBS Powder
|
Pouch for 1L x 5
|
Tween-20 (50%)
|
1ml (50%) x 5
|
30X DAB Solution
|
15 ml
|
Substrate solution (30% H2O2)
|
1ml
|
Secondary antibody (goat anti-mouse IgG-HRP)*/**
|
0.1ml
|
Secondary antibody (goat anti-rabbit IgG-HRP)*/**
|
0.1ml
|
* The dilution rate of secondary antibodies for WB is 1:1,000-1:10,000. They are also applicable to use for ELISA (1:10,000-1:100,000) as well as IHC (1:200-1:500).
** For separate purchasing, this small volume is not available.
|
|
|
Western Blot
Immunoblot Analysis Marker (lane 1),
10 ug (lane 2),
1 ug (lane 3),
0.1 ug (lane 4),
- ug (lane 5)
- of BSA samples were resolved by lectrophoresis, blotted to nitrocellulose, and probed with Anti-BSA (1:4,000). Proteins were
visualized with goat anti-rabbit secondary antibody conjugated to HRP (1:5,000) and DAB substrate. Arrow indicates BSA (69 kDa).
|
|
Dot Blot
10 ug (lane 1), 1 ug (lane 2), 0.1 ug (lane 3), 0.01 ug (lane 4), 0.001 ug (lane 5) of BSA samples were
dotted to nitrocellulose, and probed with Anti-BSA (1:4,000). Proteins were visualized with goat anti-rabbit
secondary antibody conjugated to HRP (1:5,000) and DAB substrate.
|
ORDERING INFORMATION
|
Cat. No.
|
Product
|
Size
|
K03350
|
EzWay™ DAB Western Blotting Kit
|
10 mini-blots
|
