NFP (Nano Fluorescent Particle)
Overview
NFP (Nano Fluorescent Particle) is a nanoparticle which has a magnetic & fluorescent properties at  the same time. It has a ferrite magnetic core and it is coated with a stable sillica shell to avoid potential toxic effects on cells. This sillica shell contains luminescent organic dyes such as rhodamine B isothiocyanate (RITC, orange color) or fluorescent isothiocyanate (FITC, green color) on the inside of the silica shell. Also, the silica shell can be easily fabricated with various desired organo-silicon compounds. Thus, the magnetic and fluorescence properties enable dual detection of the silica-coated core-shell nanoparticles. The additional advantage provided by the incorporation of a fluorescent dye into the silica shell is the significant increase in photochemical stability, resulting in minimal photobleaching even after multiple exposures, which is currently a topic of great importance among researchers using confocal laser scanning microscopy (CLSM). It was also reported that the organic dye encapsulated in the silica core-shell architecture results in increased fluorescence intensity due to "caging effects". Therefore, fluorescence hybrid core-shell is an attractive candidate for various biomedical applications. NFP remains stable in physiological buffers for long periods of time. By various surface modification of sillica shell, it represents different kinds of functional groups for various applications such as drug or gene delivery, specific targeting, antibody conjugation and so on.
SPECIFICATION
Magnetic and fluorescent substances The core of NFP is made up of nano-particles that are made from heavy metals of magnetic nature called ferrite. It is coated with silica (SiO2, the compound approved by F.D.A., U.S.A. in its safety) and organic fluorescent materials which are included in the inner part of silica so as to include both magnetic and optical features (Red color: RITC 543/554nm, Green color: FITC 492/518nm).  Size of NFP The size of NFP is variable from 20nm to 200nm depending on the thickness of the sillica shell, and the crystallinity and magnetic properties of the core material does not change upon coating with silica.  Monitoring of the cell movement Incorporation of the fluorescent dyes into the nanoparticles enabled us to monitor the movement of cells doped with magnetic nanoparticles under an external magnetic field by optical microscopy.  Non-toxicity & Safety Nanoparticles have been coated with a shell of stable and biocompatible material such as silica (SiO2) to avoid potential toxic effects of ferrite on cells, so it does not reveal their toxicity in vitro and in vivo test. Also, the sillica is stable at the strong basic and acidic conditions, so the internal magnetic & fluorescent properties remain stable. It is available to use to get an in vivo imaging by MRI detection.  Photostability & High signal Incorporation of a fluorescent dye into the silica shell is the significant increase in photochemical stability, resulting in minimal photobleaching even after multiple exposures.  Gene or drug delivery carrier Positive charged nanoparticles are possible to apply as gene or drug delivery carrier. And it can overcome carrier on a commercial scale limitation in safety, and efficiency. A uniform ionic surface of the nanoparticle shows more than 95% transfection efficiency.  Stem Cell Research The magnetic & fluorescent properties of NFP are expected to be also used for Stem cell studies such as detection of stem cell differenciation, in vivo distribution, stem cell sorting or imaging etc. NFP is easily uptaken by stem cells and remains within the cell, so it helps to distinguish stem cells from others as well as monitor the continuous image of stem cells.  Various Applications by various surface treatment NFP is able to be used for various applications by modification of the surface with several different surface chemistries. Various trialkoxysilane derivatives are easily introduced on the silica shell surface by a base-catalyzed condensation reaction with surface SI-OH functional groups.
NFP BASED ASSAYS
(1) Transfection / Gene or drug delivery carrier Positive charged nanoparticles are possible to apply as gene or drug delivery carrier. And it can overcome carrier on a commercial scale limitation in safety, and efficiency. A uniform ionic surface of the nanoparticle shows more than 95% transfection efficiency.
(2) Stem Cell Staining NFP is easily uptaken by stem cells and remains within the cell, so it helps to distinguish stem cells from others as well as continuous image monitoring of stem cells.
(3) Antibody Conjugation for Cell Sorting
(4) Cell Targeting
(5) In vivo Imaging
(6) MRI Detection
Cat.No Description Functional Group Magnetic core Size Conc. K2501502 NFP Silanol FITC 50 Silanol No 50nm 2mg/ml K2501505 NFP Silanol FITC 50 Silanol No 50nm 5mg/ml K2503502 NFP Silanol RITC 50 Silanol No 50nm 2mg/ml K2503505 NFP Silanol RITC 50 Silanol No 50nm 5mg/ml K2505502 NFP Silanol Magna FITC 50 Silanol Yes 50nm 2mg/ml K2505505 NFP Silanol Magna FITC 50 Silanol Yes 50nm 5mg/ml K2507502 NFP Silanol Magna RITC 50 Silanol Yes 50nm 2mg/ml K2507505 NFP Silanol Magna RITC 50 Silanol Yes 50nm 5mg/ml K2521502 NFP PEG/Amine FITC 50 PEG/Amine No 50nm 2mg/ml K2521505 NFP PEG/Amine FITC 50 PEG/Amine No 50nm 5mg/ml K2523502 NFP PEG/Amine RITC 50 PEG/Amine No 50nm 2mg/ml K2523505 NFP PEG/Amine RITC 50 PEG/Amine No 50nm 5mg/ml K2525502 NFP PEG/Amine Magna FITC 50 PEG/Amine Yes 50nm 2mg/ml K2525505 NFP PEG/Amine Magna FITC 50 PEG/Amine Yes 50nm 5mg/ml K2527502 NFP PEG/Amine Magna RITC 50 PEG/Amine Yes 50nm 2mg/ml K2527505 NFP PEG/Amine Magna RITC 50 PEG/Amine Yes 50nm 5mg/ml K2531502 NFP PEG FITC 50 PEG No 50nm 2mg/ml K2531505 NFP PEG FITC 50 PEG No 50nm 5mg/ml K2533502 NFP PEG RITC 50 PEG No 50nm 2mg/ml K2533505 NFP PEG RITC 50 PEG No 50nm 5mg/ml K2535502 NFP PEG Magna FITC 50 PEG Yes 50nm 2mg/ml K2535505 NFP PEG Magna FITC 50 PEG Yes 50nm 5mg/ml K2537502 NFP PEG Magna RITC 50 PEG Yes 50nm 2mg/ml K2537505 NFP PEG Magna RITC 50 PEG Yes 50nm 5mg/ml K2541202 NFP Amine FITC 20 Amine No 20nm 2mg/ml K2541205 NFP Amine FITC 20 Amine No 20nm 5mg/ml K2543202 NFP Amine RITC 20 Amine No 20nm 2mg/ml K2543205 NFP Amine RITC 20 Amine No 20nm 5mg/ml K2545402 NFP Amine Magna FITC 40 Amine Yes 40nm 2mg/ml K2545405 NFP Amine Magna FITC 40 Amine Yes 40nm 5mg/ml K2547402 NFP Amine Magna RITC 40 Amine Yes 40nm 2mg/ml K2547405 NFP Amine Magna RITC 40 Amine Yes 40nm 5mg/ml K2551202 NFP Active Ester FITC 20 Active Ester No 20nm 2mg/ml K2553202 NFP Active Ester RITC 20 Active Ester No 20nm 2mg/ml K2555402 NFP Active Ester Magna FITC 40 Active Ester Yes 40nm 2mg/ml K2557402 NFP Active Ester Magna RITC 40 Active Ester Yes 40nm 2mg/ml K2561502 NFP PTMA FITC 50 PTMA No 50nm 2mg/ml K2563502 NFP PTMA RITC 50 PTMA No 50nm 2mg/ml K2575502 NFP PMP Magna FITC 50 PMP No 50nm 2mg/ml K2577502 NFP PMP Magna RITC 50 PMP No 50nm 2mg/ml