Reactivity:
Articlecode: 03-62009
Product: Neurofilament 160
Catalog #: 03-62009 (250 mg)
03-62209 (100 mg)
Amount: 250 mg or 100 mg
SOURCE: Bovine spinal cord
MOLECULAR WEIGHT: 160,000
ISOELECTRIC POINT: pHI 5.1
PURITY: Greater than 98%, determined by SDS-PAGE
FORM: Lyophilized, salt balanced
APPLICATION: Protein standard in 1D and 2D SDS-gel electrophoresis
Immunoassay
Antigen for immunization.
RECONSTITUTION (250 µg): When reconstituted with 200µl H20 dist, a final solution volume of 250 µl will contain 10mM sodium phosphate, pH 7.5, 2mM DTT, 6M urea, 1mM EDTA with a protein concentration of 1mg/ml (according to Bradford protein determination)
RECONSTITUTION (100 µg): When reconstituted with 80µl H20 dist, a final solution volume of 100 µl will contain 10mM sodium phosphate, pH 7.5, 2mM DTT, 6M urea, 1mM EDTA with a protein concentration of 1mg/ml (according to Bradford protein determination)
STORAGE: at 4°C (lyophilized); at -20°C (solution, aliquot)
REFERENCES:
Dahl, D.et al. (1982). Analyt. Biochem. 126: 165ff
Weber, K et al. (1983) Quant. Biol. 48: 717ff
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