Reactivity: rat
Articlecode: 03-16051
Product: Anti-Neurokinin A/Substance K
Catalog #: 03-16051
Amount: 50 ml
CATEGORY: Rabbit polyclonal
IMMUNOGEN: Neurokinin A/Substance K (Peninsula), conjugated to BSA
FORM: Serum containing 0.09% sodium azide
DESCRIPTION/SPECIFICITY: Neurokinin A/Substance K represents a member of the tachykinin family of peptides. Neurokinin A, which arises by cleavage of the substance P precursor, occurs in neurons in the central and peripheral nervous systems, and is particularly numerous in the gastrointestinal tract. The biological actions of neurokinin A are similar to those of subastance P, and include vasodilation and stimulation of smooth muscle contraction. Absorption with 10-100 mg NKA per ml diluted antiserum abolishes the staining, while substance P does not.
POSTIVE CONTROL: Formalin-fixed paraffin sections of rat colon.
SPECIES TESTED SO FAR: Rat
APPLICATION (tested so far): - Immunofluorescence
- Immunohistochemistry, suitable for frozen and paraffin sections
WORKING DILUTION 1:20 - 1:40 for immunofluorescence microscopy (FITC) with overnight incubation at 2-8°C
1:40 - 1:80 (PAP)
DILUTION BUFFER: 0.1 M PBS containing 1% BSA and 0.1% NaN3.
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot) for extended storage
REFERENCE
Lundberg LM. Growth and degeneration of uterine innervation. An experimental study in the guinea pig. Thesis Lund 1988
Schmidt et al.. (1991) Peptides 12: 963-973
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