Format: purified - Reactivity: human, rat, mouse - Synonym: nNOS, NOS type I, Constitutive NOS, neur
Articlecode: NP2141
Clone:
Isotype: Rabbit polyclonal
Applications: WB, ELISA
WB: 1:1000 ELISA: 1:2000 IP: IC:
Synonym: nNOS, NOS type I, Constitutive NOS, neuronal nitric oxide synthase, NC-NOS
BACKGROUND:
Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products.
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