Reactivity: mouse
Articlecode: 24-6800-92
Contents: Serum anti-mouse Neuropilin-1
Sizes: 100 ul
Storage Conditions: Store at 4°C.
Clone: Polyclonal
Isotype: Goat IgG
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Available Formats of This Product
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Cat. No.
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Format
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Excite
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Emit
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Reported Applications
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24-6800
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N/A
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N/A
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Description
The Neuropilin-1 antibody reacts with mouse Neuropilin-1, a cell surface glycoprotein originally identified in Xenopus tadpole nervous tissues. Immunohistochemistry and in situ hybridization analyses in mice indicated that the expression of neuropilin was restricted to axons of particular neuron classes, and at stages when axon growth is active. Neuropilin protein was localized on axons but not on the somata of neurons and can mediate cell adhesion by heterophilic molecular interaction. The gain and loss of function of neuropilin in developing mouse embryos causes defasciculation and incorrect sprouting of nerve fibers. It is postulated that neuropilin serves in a variety of neuronal cell interactions by binding to a variety of molecules, and that it plays essential roles in nerve fiber fasciculation and targeting.
Usage
For research use only, not for diagnostic or therapeutic use. The Polyclonal antibody has been reported for use in immunohistochemical staining of frozen mouse embryonic tissue sections (Fixation with 4% PFA).
Applications Tested
The Polyclonal antibody has been tested by immunohistochemical staining of embryonic mouse neural tissues (spinal motor axons and DRG sensory axons) and embryonic blood vessels. The serum should be diluted 1:300-1:1000 in PBS.
References
Bruder, D., et al. 2004. Neuropilin-1: a surface marker of regulatory T cells. Eur. J. Immunol. 34: 1498.
Kawakami, A., et al. 1996. Developmentally regulated expression of a cell surface protein, neuropilin, in the mouse nervous system. J Neurobiol. 29:1-17.
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