Format: Supernatant; Reactivity: human, Xenopus
Articlecode: 03-651124
Product: Anti-Nucleolar Protein xNopp180
Catalog #: 03-651124
Amount: 1 ml
CATEGORY: Mouse monoclonal
CLONE DETERMINATION: No-114
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Nucleolar fraction prepared from Xenopus laevis oocytes
FORM: Hybridoma culture supernatant
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Xenopus laevis, X. borealis, XLKE-A6 cells; human (see below). Negative with Pleurodelis, Triturus, Rana, chicken, rat, mouse
CLINICAL APPLICATION/SPECIFICITY: The antibody stains specifically the nucleolus and Cajal bodies
in therphase cells. The antigen is a constitutively expressed nucleolar component and a specific marker for the dense fibrillar component of the interphase nucleolus. The protein recognizes is a 180 kD polypeptide in Xenopus (xNopp180), homologue to the rat polypeptide (Nopp140). When applied at high concentration to human cells the antibody reacts with a nucleolar component which, however, does not correspond to xNopp180.
APPLICATION: Immunoblotting (western)
Immunoprecipitation
Immunofluorescence microscopy on frozen sections (also after fixation with paraformaldehyde)
WORKING DILUTION: 1:5 to 1:10 for Immunofluorescence
1:50 for immunoblotting (western, ECL method)
INCUBATION TIME: 1 h at room temperature
STORAGE: 2-8°C for immediate use or -20°C (aliquot)
REFERENCES:
Schmidt-Zachmann, MS et al. (1984) Exp. Cell Res. 153: 327-346
Cairns, C. et al. (1995) J. Cell Sci. 108: 3339-3347
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