MoAb
Articlecode: 01--2465
Product: Anti-NPM
Catalog #: 01-2465
Amount: 200 ml
CATEGORY: Mouse monoclonal
CLONE DETERMINATION: 5E3
IMMUNOGLOBULIN CLASS: Mouse IgG2b heavy chain and kappa light chain
FORM: Purified, in PBS, pH 7.4, containing 0.1% sodium azide
IMMUNOGEN: Recombinant human NPM (81-294aa) purified from E. coli
PURIFICATION: Protein G affinity chromatography, from ascites
DESCRIPTION/SPECIFICITY: Nucleophosmin (NPM; also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage.Nucleophosmin is upregulated in many cancer cell
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Human
APPLICATION (tested so far): ELISA, Immunoblotting (western, ECL)
WORKING DILUTION: Western Blot: 1:100-1:200
Optimal dilution should be performed by serial dilution
STORAGE: 2-8°C for immediate use; or at -20°C (aliquot)
REFERENCES:
Aliprantis AO et al. (1999) Science 285:736-739
Matsuguchi T et al., (2000) J Immunol. 165(10): 5767-5772
Takeda K et al., (2003) annurev. Immunol. 21:335-376
Janeway, C. A. et al., (2002) Annu. Rev. Immunol. 20:197
Yang RB, et al., (1998) Nature 395(6699) 284-288
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