Format: purified - Reactivity: human, rat, rabbit - Synonym: Protein Kinase C alpha
Articlecode: PP1091
Clone:
Isotype: Rabbit polyclonal
Applications: WB, ELISA, IP
WB: 1:1000 ELISA: 1:2000 IP: 5-10ml IC:
Synonym: Protein Kinase C alpha
BACKGROUND:
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (alpha, beta I, beta II, and gamma) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKC beta I and PKC alpha, respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.
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