Format: purified - Reactivity: human, rat, mouse - Synonym: Phospholipase C gamma 1, 1-phosphatidyli
Articlecode: PP1491
Catalog Number: PP1491
Clone:
Isotype: Rabbit polyclonal
Applications: WB, ELISA, IP, IC
WB: 1:1000 ELISA: 1:2000 IP: 1-5ml IC: 1:100
Synonym: Phospholipase C gamma 1, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
BACKGROUND:
Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLCs have been identified: PLC beta, PLC gamma, PLC delta, and PLC epsilon. Phosphorylation is one of the key mechanisms that regulates the activity of PLC. PLC delta is activated by both receptor and nonreceptor tyrosine kinases. PLC gamma 1 forms a complex with EGF and PDGF receptors, which leads to phosphorylation at tyrosine 771, 783, and 1245. In addition, antigen receptor-induced activation of PLC gamma 1 leads to phosphorylation at both Tyr-775 and Tyr-783. These two sites are equally important for activation of enzymatic activity.
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