Diagnostic Test - Molecular Biology
Catalog No.: 22212
The GenoType® PAI- test is based on the DNA•STRIP® technology and permits the combined molecular characterization of the positions –675 and –844 in the promoter region of the human plasminogen activator inhibitor 1 (PAI-1) gene.
The whole procedure is divided into three steps: DNA isolation from a patient sample (blood; the necessary DNA isolation kit is not provided), a multiplex amplification with biotinylated primers (the necessary thermostable DNA polymerase is not provided), and a reverse hybridization.
The hybridization includes the following steps: chemical denaturation of the amplification products, hybridization of the single-stranded, biotin-labelled amplicons to membrane-bound probes, stringent washing, addition of a streptavidin/alkaline phosphatase (AP) conjugate, and an AP mediated staining reaction. A template ensures the easy and fast interpretation of the banding pattern obtained.
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