RANTES Core Kit, human

Cat. No.	K0131221
Product	Human RANTES ELISA Core Kit
Type	ELISA Kit
Quantity	1000 well assay
Specificity	Human RANTES ELISA Core kit contains 5 key components required for the quantitative measurement of natural and/or recombinant Human RANTES in serum or other biological samples in a sandwich ELISA format within the range of 32-3000 pg/ml.
Description	Using the ELISA protocol described below, the components supplied in this kit are sufficient to assay Human RANTES in approximately 1000 ELISA plate wells.
Kit Components	Coating Antibody : Rabbit anti-Human RANTES Detection Antibody : Biotinylated rabbit anti-Human RANTES Standard Protein : Recombinant Human RANTES Avidin-HRP conjugate TMB Substrate Kit
Materials Required	ELISA Microtiter Wells Plate Coating Buffer (50mM Carbonate-Bicarbonate Buffer, pH9.6) Blocking Solution (0.1% Casein/PBS) Washing Solution (Mixture of PBS and Tween 20) Stop Solution (2M H2SO4) * Using the ELISA starter kit (BSA or Casein) is recommended.
Dinamic Range	32 pg/ml to 3000 pg/ml
Cross Reactivity	Human
Storage & Shelf life	1 year at 2-8°C
Sandwich ELISA Protocol	1. Coating Dilute capture antibody with DW and add to each ELISA plate well. Incubate for at least 1 hr at room temperature. (or overnight at 4°C). After incubation, remove the solution from each well and wash the plate 3-5 times. Make sure that residual buffer in each well was removed. 2. Blocking Add 200ul Blocking Solution to each well. Incubate for at least 1 hr at room temperature.After incubation, remove the solution from each well and wash the plate 3-5 times. 3. Reaction Dilute standard or sample in Diluent and add to each well. Incubate for at least 1 hr at room temperature. After incubation, remove the standard or sample from each well and wash the plate 3-5 times. 4. Detection Dilute detection antibody in diluent and add 100ul per well. Incubate for at least 1 hr at room temperature. After incubation, remove the solution from each well and wash the plate 3-5 times. 5. Enzyme Conjugate Dilute Avidin-HRP at 1:2000 in diluent and add 100ul per well. Incubate for at least 1 hr at room temperature. After incubation, remove the solution from each well and wash the plate 3-5 times. 6. Color Reaction and Reading Mix 50ul TMB solution and 100ul Substrate solution prior to use. Add this mixture to each well. After sufficient color development (5-10minutes at room temperature or at 37°C), add 50 ul Stop Solution (2M H2SO4) to each well. Using a microtiter plate reader, read the plate at the wavelength that is appropriate for the substrate used (450 nm for TMB).