Format: Purified; Reactivity: human, Insect cells
Articlecode: 03-61223
Product: Anti-RNA Polymerase II
Catalog #: 03-61223
Amount: 25 ml
CATEGORY: Mouse monoclonal
CLONE: ARNA-3
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Purified RNA Polymerase II
FORM: Ascites containing 0.09% sodium azide
DESCRIPTION/SPECIFICITY: ARNA-3 is excellent for detection of transcription activity, e.g. puffs in plytene chromosomes. It reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological
materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band
of phosphorylated Pol IIA.
Epitope recognized: 794DDYGPESRGFVENSYLA822
Reactivities on cultured cell lines: Drosophila chromosomes, PTK-2, HeLa, Hep2
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Human, insects
APPLICATION: Immunoblotting (Western)
Immunohistochemistry on frozen tissue
Cell Culture
WORKING DILUTION: Immunohistochemistry: 1:500
Recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min
INCUBATION TIME: 1 hour at room temperature for immunohistochemistry
STORAGE: 2-8°C for immediate use, or -20°C (aliquot)
REFERENCES:
Kramer, A. et al. (1980) Molec. Gen. Genet. 180: 193-199
Petersen, G. et al. (1995) Molec. Gen. Genet. 249: 425-431
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