Format: Supernatant; Reactivity: human
Articlecode: 08-9433-1
Product: Anti-Rh D antigen
Catalog #: 08 - 9433-1 Culture Supernatant
Amount: 0.5 ml Culture Supernatant
CLONE DETERMINATION: BRAD-3. It is produced by an EBV transformed B cell line derived from the peripheral blood of an immunized Rh D negative donor. This monoclonal anti-D reacts as an indirect
agglutinin with all Rh D positive red cells tested except those of the rare DVI or Ro
har types. By Flow Cytometry, FITC-BRAD-3 can be used to quantitate accurately the numbers of Rh D positive cells in a mixture of Rh D positive and negative cells, and thereby estimate the size of feto-maternal bleeds.
FORM: Liquid in 0.1m glycine and 1M Tris buffer, pH 7.0. Sterile filtered
No preservative.
IMMUNOGLOBULIN CLASS: Human IgG3
FORM: Culture supernatant, purified, FITC or PE- conjugate
ANTIGEN DISTRIBUTION: In human the Rh D antigen is expressed solely on the erythrocytes of Rh D positive individuals. 85% of Caucasians are Rh D positive.
STORAGE: 2-8°C
REFERENCES:
Cartron, J-P, (1994) Blood Reviews 8, 199
Jones et al., (1996), Vox Sanguinis 71, 176-183
Kumpel et al., (1989) Br. J. Haematol. 71, 125
Lloyd-Evans et al., (1995), Transfusion Medicine 5, 171
Lloyd-Evans et al., (1996). Transfusion Medicine 36, 432-437
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