Format: Supernatant; Reactivity: human
Articlecode: 03-57040
Product: Anti-Ro60 (SS-A) Protein (Human autoantigen)
Catalog #: 03-57040
Amount: 200 ml
CATEGORY: Mouse monoclonal
CLONE: 2G10
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Recombinant Ro60 protein
FORM: Cell culture supernatant
DESCRIPTION/SPECIFICITY: Mab 2G10 reacts with the human Ro60 (SS-A) antigen. It does precipitate the so-called Ro ribonucleoprotein complexes.
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Human
APPLICATION: Immunoblotting (Western)
Immunofluorescence Microscopy
Immunoprecipitation of Ro ribonucleoprotein complexes
RECOMMENDED FOR POSITIVE CONTROL: HEp-2 cells
WORKING DILUTION: Immunoblotting: 1:5 to 1:10, optimal dilution should be determined by serial dilution
Immunofluorescence microscopy: 1:5 to 1:10
DILUTION BUFFER: PBS containing 1% BSA and 0.09% sodium azide
STORAGE: 2-8°C for immediate use; or at -20°C (aliquot)
REFERENCES:
1. Manual of Biological Markers of Disease (1993) Section A (Eds. W.J. van Venrooij & R.N. Maini),
Kluwer Acad. Publ. Dordrecht
2. Manual of Biological Markers of Disease (1994), Section B-4.1 (Eds. W.J. van Venrooij & R.N. Maini),
Kluwer Acad. Publ. Dordrecht
3. Slobbe et al. (1991) Ann. Med. Interne. 142: 592-600
4. Veldhoven, CHA. et al. (1992) J. Immunol. Methods 151: 177-189
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