Reactivity: human
Articlecode: 03-16100
Product: Anti-S-100
Catalog #: 03-16100
Amount: 100 ml
CATEGORY: Rabbit Polyclonal
FORM: Serum containing 0.1% sodium azide
IMMUNOGEN: S-100 protein from bovine brain
DESCRIPTION/SPECIFICITY: Reacts with the 21-24 kD (the Aand B subunits) of the S-100 protein. S-100A is composed of one alpha- and one beta-chain, whereas S-100B is composed of two beta-chains. S-100 is a brain protein normally present in astrocytes, melanocytes ependymal cells, glial cells and Schwann cells.
Langerhans cells in the skin and interdigitating reticulum cells in the paracortex of lymph nodes also stain positively. S-100 occurs in tumors of the central nervous s ystem, such as Schwannomas, ependymomas, astrogliomas, gliastomas and almost all melanomas.
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Human
RECOMMENDED FOR POSITIVE CONTROL: Human skin
APPLICATION: Immunohistochemistry, suitable for frozen and paraffin sections*.
*no pretreatment erquired; Immunofluorescence
WORKING DILUTION: 1:50 to 1:100 immunohistochemistry with overnight incubation at 2-8°C.
Dilute immediately before use with PBS
DILUTION BUFFER: PBS with 0.5% BSA and 0.1% Na-Azide
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
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