Format: serum - Reactivity: human
Articlecode: 03-GP34
Product: Anti-S3-12, C-terminal
Catalog #: 03-GP34
Amount: 25 ml
CATEGORY: Guinea pig Polyclonal (GP34 hCT B)
FORM: Stabilized Antiserum, containing 0.1% sodium azide
IMMUNOGEN: Synthetic Peptide of human S3-12 (aa 1394-1410, C-terminal) conjugated to KLH (Sequence: C-QGLEQLLEGLQHNPPLS)
DESCRIPTION/SPECIFICITY: Specific for S3-12, 140 kDa (SDS-PAGE).
S3-12 pertains to PAT-family proteins, covering the surface of Cytoplasmic lipid droplets Additional PAT-family proteins include adipophilin (ADRP), perilipin, TIP47, and MLDP (OXPAT/PAT-1 or LSDP5) which are expressed in differentiation related stages of lipid metabolism
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Human
REACTIVITIES IN CULTURE
CELL LINES (tested so far): PLC (human)
APPLICATION: Immunofhistochemistry on cells and frozen tissues
Immunoblotting (western, ECL)
Cytological material
WORKING DILUTION: 1:100 - 1:200 for Immunofhistochemistry applications
1:2000 for Immunoblotting (western, ECL)
INCUBATION TIME: 1 hour at room temperature (extended with paraffin)
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
Wolins, NE. et al. (2005) J Biol Chem 280: 19146-19155
Dalen, KT et al. (2004) Diabetes 53: 1243-1252
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