Reactivity: human
Articlecode: 14-6769-81
Contents: Affinity Purified anti-human SIRT1 polyclonal
Catalog Number: 14-6769
Sizes: 10 ug, 50 ug
Formulation: 200 µg/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG
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Description
The polyclonal antibody reacts with SIRT1; the antibody was raised against a peptide mapping to the carboxy terminal domain of human SIRT1. The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4 (1). The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53 (1-3). SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras (2&3). The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress (1-3).
Usage
For research use only, not for diagnostic or therapeutic use. Purified anti-human SIRT1 poly has been reported for use in immunoblotting (WB).
Applications Tested
Purified anti-human SIRT1 poly has been tested by immunoblotting (WB). (1:400 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.
Related Products
Cat. 18-8816 Rabbit TrueBlot™: Horseradish Peroxidase (HRP) anti-rabbit IgG
Cat. 66-P769 Blocking Peptides for anti-human SIRT1 polyclonal
References
1. Smith J. 2002. Human Sir2 and the 'silencing' of p53 activity. Trends Cell Biol. 12(9):404-406.
2. Vaziri H, Dessain SK, Ng Eaton E, Imai SI, Frye RA, Pandita TK, Guarente L, Weinberg RA. 2001. HSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Cell 107(2):149-159.
3. Langley E, Pearson M, Faretta M, Bauer UM, Frye RA, Minucci S, Pelicci PG, Kouzarides T. 2002. Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence. EMBO J. 21(10):2383-2396.
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