Format: Supernatant; Reactivity: human
Articlecode: 03-57030
Product: Anti-Sm D1 Protein (Human autoantigen)
Catalog #: 03-57030
Amount: 200 ml
CATEGORY: Mouse monoclonal
CLONE: ANA 127
IMMUNOGLOBULIN CLASS: IgA
IMMUNOGEN: Spleen cells from an MRL/lpr mouse
FORM: Cell culture supernatant
DESCRIPTION/SPECIFICITY: Mab ANA 127 reacts with the Sm D1 protein, contained in major snRNPs (U1, U2, U4/U6 and U5 snRNPs).
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Human
APPLICATION: Immunoblotting (Western)
Immunofluorescence microscopy
Immunoprecipitation
RECOMMENDED FOR POSITIVE CONTROL: HEp-2 cells
WORKING DILUTION: Immunoblotting: 1:5 to 1:10, optimal dilution should be determined by serial dilution
Immunofluorescence microscopy: 1:5 to 1:10
DILUTION BUFFER: PBS containing 1% BSA and 0.09% sodium azide
STORAGE: 2-8°C for immediate use; or at -20°C (aliquot)
REFERENCES:
Manual of Biological Markers of Disease (1993) Section A (Eds. W.J. van Venrooij & R.N. Maini), Kluwer Acad. Publ. Dordrecht
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