Reactivity: human
Articlecode: 14-6742-63
Contents: Affinity Purified anti-human Smac/Diablo polyclonal
Catalog Number: 14-6742
Sizes: 10 ug, 50 ug
Formulation: Smac/DIABLO (C17) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of human Smac/DIABLO.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG
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Available Formats of This Product
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14-6742
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Description
The polyclonal antibody reacts with Smac/DIABLO; the antibody was raised against a peptide mapping to the carboxy terminus of human Smac/DIABLO. Second mitochondrial activator of caspases or Smac/DIABLO can promote apoptosis by relieving the inhibition of caspases by the IAPs (1&2). The other mitochondrial activator of apoptosis is cytochrome c (1). Smac/DIABLO export from the mitochondria into the cytosol can be triggered by cytotoxic drugs, DNA damage, and the ligation of FAS (CD95) death receptor (3). Once in the cytosol Smac/DIABLO eliminates the inhibitory effects of the IAPs through and amino terminal interaction with the IAP proteins, and thus allow the activation of caspases (2&4). The interaction of Smac/DIABLO?s amino terminus with the IAP proteins has been localized to their BIR domains, for XIAP the key domain is BIR3 (4&5).
Usage
For research use only, not for diagnostic or therapeutic use. Purified anti-human Smac/Diablo poly has been reported for use in immunoblotting (WB).
Applications Tested
Purified anti-human Smac/Diablo poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.
Related Products
Cat. 18-8816 Rabbit TrueBlot™: Horseradish Peroxidase (HRP) anti-rabbit IgG
Cat. 66-P742 Blocking Peptides for anti-human Smac/Diablo polyclonal
References
1. Du C., Fang M., Li Y., Li L., Wang X. 2000. Smac, a Mitochondrial Protein that promotes Cytochrome c-Dependent Caspase Activation by Eliminating IAP Inhibition. Cell 102:33-42.
2. Springs S.L., Diavolitsis V.M., Goodhouse J., Mclendon G.L. 2002. The kinetics of translocation of Smac/DIABLO from the mitochondria to the cytosol in HeLa cells. J Biol Chem
3. Adrain C., Creagh E.M., Martin S.J. 2001. Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2. EMBO J 20(23):6627-6636.
4. Wu G., Chai J., Suber T.L., Wu J.W., Du C., Wang X., Shi Y. 2000. Structural basis of IAP recognition by Smac/DIABLO. Nature 408(6815):1008-1012.
5. Liu Z., Sun C., Olejniczak E.T., Meadows R.P., Betz S.F., Oost T., Herrmann J., Wu J.C., Fesik S.W. 2000. Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain. Nature 408(6815):1004-1008.
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