Reactivity: human
Articlecode: 14-6762-81
Contents: Affinity Purified anti-human Sp17 polyclonal
Catalog Number: 14-6762
Sizes: 10 ug, 50 ug
Formulation: 200 µg/ml rabbit polyclonal IgG in PBS containing 0.1 % sodium azide and 0.2% gelatin.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG
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14-6762
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Description
The polyclonal antibody reacts with Sp17; the antibody was raised against a peptide mapping to the carboxy terminal domain of human Sp17. The highly immunogenic cancer-testis antigen Sp17 was originally thought to be expressed exclusively in the testis with the primary function of binding the extra cellular matrix of the oocyte (1,2). Upon further investigation Sp17 was found to be expressed by tumor cells with multiple myeloma in up to 30% of the cases studied (3,4). Sp17 has three functional domains, N-terminal, central, and C-terminal domains. The N-terminal domain contains an A-kinase anchoring protein binding motif. The central domain is necessary for heparin binding and the C-terminal domain contains a calmodulin-binding domain (1&2).
Usage
For research use only, not for diagnostic or therapeutic use. Purified anti-human Sp17 poly has been reported for use in immunoblotting (WB).
Applications Tested
Purified anti-human Sp17 poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.
Related Products
Cat. 18-8816 Rabbit TrueBlot™: Horseradish Peroxidase (HRP) anti-rabbit IgG
Cat. 66-P762 Blocking Peptides for anti-human Sp17 polyclonal
References
1. Wen Y, Richardson RT, Widgren EE, O?Rand MG. 2001. Characterization of Sp17: a ubiquitous three domain protein that binds heparin. Biochem J. 357(Pt1):25-31.
2. Frayne J, Hall L. 2002. A re-evaluation of sperm protein 17 (Sp17) indicates a regulatory role in an A-kinase anchoring protein complex, rather than a unique role in sperm pellucida binding. Reproduction 124(6):767-774.
3. Chiriva-Internati M, Wang Z, Salati E, Wroblewski D, Lim SH. 2002. Succuessful generation of sperm protein 17 (Sp17)-specific cytotoxic T lymphocytes from normal donors: implication for tumour-specific adoptive immunotherapy following allogeneic stem cell transplantion for Sp17-positive multiple myeloma. Scand J Immunology 56(4):429-433.
4. Chiriva-Internati M, Wang Z, Xue Y, Bumm K, Hahn AB, Lim SH. 2001. Sperm protein (Sp17) in multiple myeloma: opportunity for myeloma-specific donor T cell infusion to enhance graft-versus-myeloma effect without increasing graft-versus-host disease risk. Eur J Immunology 31(8):2277-2283.
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