Reactivity: human, mouse, rat
Articlecode: 14-6013-63
Contents: Anti-mouse/human/rat Stat3 (C-terminus) polyclonal
Catalog Number: 14-6013
Sizes: 10 ug, 50 ug
Storage Conditions: This product is supplied as purified rabbit IgG fraction in PBS containing 0.2% BSA and 0.02% NaN3. Store at 4°C, stable for 6 months. Stable at -20°C for one year. Avoid repeated freeze/thaw cycles.
Clone: Polyclonal
Isotype: Rabbit IgG
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Available Formats of This Product
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Format
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Emit
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Reported Applications
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14-6013
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N/A
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N/A
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Description
The polyclonal antibody reacts with human, mouse, and rat Stat3; the antibody was raised against a synthetic peptide from the C-terminus of human Stat3, a transcription factor expressed in most cell types, which is activated by multiple cytokines and growth factors. These include IFN-ã, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, RGF, PDGF and CSF-1. Stat3 binds to DNA as either a homodimer or as a heterodimer with Stat1. Stat3 interacts with APRE (acute phase response element) and SIE (c-sis inducible element). Stat3 has an expected molecular weight of 89 kDa. Cross-reactivity with other species is unknown.
Usage
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).
Applications Tested
This polyclonal antibody has been tested by immunoblotting (5-10 ìg/ml). Jurkat cells may be used as a positive control. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 18-8816 Rabbit TrueBlot™: Horseradish Peroxidase (HRP) anti-rabbit IgG
References
Daino H, Matsumura I, Takada K, Odajima J, Tanaka H, Ueda S, Shibayama H, Ikeda H, Hibi M, Machii T, Hirano T, Kanakura Y. 2000. Induction of apoptosis by extracellular ubiquitin in human hematopoietic cells: possible involvement of STAT3 degradation by proteasome pathway in interleukin 6-dependent hematopoietic cells. Blood. Apr 15;95(8):2577-85.
Frank DA. 1999. STAT signaling in the pathogenesis and treatment of cancer. Mol Med. Jul;5(7):432-56.
Stephanou A, Latchman DS. 1999. Transcriptional regulation of the heat shock protein genes by STAT family transcription factors. Gene Expr. 7(4-6):311-9.
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