Contents: Carrier-Free Recombinant Mouse Stem Cell Factor (SCF)
Catalog Number: 34-8341
Sizes: 100 ug, 500 ug
Formulation: Sterile liquid; 10 mM sodium phosphate, 150 mM NaCl, pH 7.2, with no carriers or stabilizers. 0.22 uM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -20°C in a non-cycling freezer (e.g., not 'frost free'). Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Lys 26-Ala 189 of mature mouse SCF (accession # M59915)
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 18,298. The DTT reduced protein migrates as an 18 kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 16 kDa polypeptide.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse MC/9 cell proliferation assay. The ED₅₀ is 15 ng/ml, corresponding to a specific activity of 6.7 x 10⁴ Units/mg.

 

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