Reactivity: -
Articlecode: 05-52151
Product: Streptavidin
Catalog #: 05-52151
Amount: 1 mg
DESCRIPTION: Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240 kDa and containing 4 subunits each with a binding site for biotin. The Streptavidin is isolated by purification from fermentation broth. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction results in a very quick reaction with a high signal to noise ratio. Streptavidin does not react unspecifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.
SOURCE: Streptomyces avidinii
ENDOTOXIN: Less than 0.1 ng/µg (IEU/µg) of rStrptavidin
ACTIVITY: > 13 U/mg, I unit binds 1 ìg biotin
FORM: Purified, in 1 mM MgCl2, 0.1 mM ZnCl2. 30 mM Triethanolamine, pH 7.6
APPLICATION: Avidin may be used to visualize biotin conjugated molecules in ELISA, Blotting and histological techniques
STORAGE: -20°C (aliquot), avoid freezing and thawing cycles
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