Reactivity: rat
Articlecode: 03-16098
Product: Anti- Rat Substance P
Catalog #: 03-16098
Amount: 100 ml
CATEGORY: Rabbit Polyclonal
FORM: Serum containing 0.1% sodium azide
IMMUNOGEN: Substance P conjugated to BSA
DESCRIPTION/SPECIFICITY: Substance P occurs in nerve fibers of the central and peripheral nervous system and in endocrine cells of the gut. It stimulates smooth muscle contraction, gives rise to vasodilation and is involved in sensory functions. Substance P-containing tumors arising in the iliem are often associated with the carcinoid syndrome, characterized by flushing of the skin, diarrhea, broncho-constriction and sudden drops in blood pressure. Substance P is commonly found in the midgut carcinoids and some of the symptoms may be related to this peptide. Absorption with 10-100 mg SP and NKA per ml diluted antiserum abolishes the staining while Neurokinin A does not.
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Rat
RECOMMENDED FOR POSITIVE CONTROL: Frozen sections of rat colon
APPLICATION: Immunohistochemistry, suitable for frozen and paraffin sections
Immunofluorescence
WORKING DILUTION: 1:20 to 1:40 for immunofluorescence microscopy, with overnight incubation at 2-8°C
DILUTION BUFFER: 0.1 M PBS with 1% BSA and 0.1% Na-Azide
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
Hardebo, JE et al. (1989) Neuroscience letters 101: 39-45
Hauser, JE. et al. (1994) J. Andrology 15 (6): 510-520
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