Format: Purified; Reactivity: human, mouse, rat, bovine
Articlecode: 03-61012
Product: Anti-Synaptophysin
Catalog #: 03-61012 Purified
Amount: 200 ml Purified
CATEGORY: Mouse monoclonal
CLONE DETERMINATION: SY 38
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Synaptophysin (presynaptic vesicles)
ANTIGEN RECOGNIZED IN SPECIES: human, bovine, rat, mouse
SPECIAL NOTE: SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors.
PURIFICATION: SpAss Protein A (recombinant)
APPLICATION: Suitable for frozen and paraffin embedded tissue
Cytological material
FACS analysis
Immunoblotting (Western).
POLYPEPTIDE REACTING: MR 38,000 transmembrane glycoprotein of presynaptic vesicles.
REACTIVIES ON CULTURED
LINES: Rat PC-12 cell line.
TUMORS SPECIFICALLY
DETECTED: Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet
cell carcinomas, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid.
PRETREATMENT: In no case protease pretreatment.
WORKING DILUTION: Dilute further to 1:50 for immunohistochemistry
INCUBATION TIME: 1 hour at room temperature, extended with paraffin (overnight at 4°C)
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
Gould, V.E.et al. (1987). Am. J. Pathol. 126: 243-257
Gould, V. E.et al. (1986). Human Pathol. 17: 979-983
Gould, V.E. (1987). Arch. Pathol. Lab. Med. 111: 791-794
Kelly, R. B.et al. (1983). Cold Spring Harbor Symp. Quant. Biol. 48: 697-705
Wiedemann, B. et al. (1986). Proc. Natl. Acad. Sci. USA 83: 3500-3504
Wiedenmann, B. and Franke, W.W. (1985). Cell 45: 1017-1028
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