Format: Supernatant; Reactivity: human. mouse. rat. bovine, Guinea pig
Articlecode: 03-65194
Product: Anti-Synaptopodin
Catalog #: 03-65194
Amount: 1 ml
CATEGORY: Mouse monoclonal
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Isolated rat kidney glomeruli
FORM: Cell culture supernatant
DESCRIPTION/SPECIFICITY:
1) The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro. Weaker reaction with arterial endothelial cells. It does not react with parietal cells.
2) It reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression
during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons.
ANTIGEN RECOGNIZED: 100/110 prolin-rich actin-associated protein of podocytes and a subset of telencephalic postsynaptic densities.
ANTIGEN RECOGNIZED IN
SPECIES: Human, bovine, rat, mouse, guinea pig,gerbil; negative with rabbit, frog, chicken.
APPLICATION: Immunohistochemistry on frozen and paraffin embedded sections.
Immunoblotting (Western)
Immunofluorescence
INCUBATION TIME: 1 hour at room temperature for IHC
WORKING DILUTION: Ready to use for immunocytochemistry
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
1. Mundel, P. et al. (1991) J. Histochem & Cytochem 39 No.8: 1047-1056
2. Mundel, P. & Kritz, W. (1995) Anat. Embryol. 192: 385-397
3. Mundel, P. et al. J Am Soc Nephrol 8: 8978-705
4. Mundel, P. et al. (1997) J. Cell Biol. 139(1): 193-204
5. Mundel, P. et al. (1997) Exp. Cell Res (in press)
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