MoAb
Articlecode: 01--2412
Product: Anti-Synuclein á (aa 61-95), human
Catalog #: 01-2412
Amount: 200 ìl
CATEGORY: Mouse Monoclonal
CLONE: 5C2
IMMUNOGLOBULIN SUBTYPE: Mouse IgG1, kappa
IMMUNOGEN: Recombinant human á-synuclein (1-140), E. coli
FORM: Affinity purified (Protein G) from mouse ascites
SPECIFICITY: Human á-synuclein only, the NAC domain
APPLICATION: ELISA, Western Blot
CONCENTRATION: 0.1mg/ml
WORKING DILUTION: Western blot (ECL) analysis is 1:100 ~ 1:400.
Recommended starting dilution is 1:100.
BUFFER: PBS (pH 7.4) with 0.1% sodium azide
STORAGE: -20°C (aliquot), Avoid repeated freeze and thaw cycles.
Western Blot Analysis:
The recombinant human synuclein family (á-, â- and ã-) and á-synuclein domains (1-60, 1-95, 61-140 and 96-140 proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-á-Synuclein (61-95 aa) antibody (1:200). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system
Reference:
Jakes, R., et al. (1994) FEBS lett. 345, 27-32
Ueda, K., et al. (1993) Proc. Natl.Acad. Sci. USA 90, 11282-11286
Kim, J. (1997) Molecules and Cells 7, 78-83
Paik, S. R., et al. (1997) Arch. Biochem. Biophys. 344, 325-334
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