Reactivity: Mouse
Articlecode: 16-5860-80
Contents: Functional Grade Purified Mouse Tim-1-Fc fusion protein
Catalog Number: 16-5860
Sizes: 25 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with no carrier or stabilizer. 0.22 uM filtered.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: Insect cells infected with baculovirus: amino acids met 1-gly 214 corresponding to the extracellular domain TIM-1 (accession #AF399830) fused with Xa cleavage site -human Fc-His6.
Molecular Mass: The intact protein corresponding to the extracellular TIM-1 domain (tyr 22 - gly 214) -Xa cleavage site-human Fc-His6 has a predicted molecular mass of 51,446. The DTT reduced protein migrates as a 65 kDa polypeptide on SDS-PAGE due to glycosylation. Some 51 kDa polypeptide is also present. On non-reducing SDS-PAGE the homodimeric cystine-linked protein migrates as a doublet at 95-120 kDa.
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
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Available Formats of This Product
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Reported Applications
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16-5860
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N/A
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FA
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg
(unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
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Description
The Tim-1-Fc is a soluble fusion protein derived from expression of a fusion of cDNA encoding the entire extracellular domain of mouse Tim-1 to a cDNA encoding a human IgG1 Fc tail. TIM-1, a member of newly identified T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM family of proteins are transmembrane proteins expressed by CD4 T cells. Recent studies demonstrate that the TIM family, particularly TIM-1, plays a critical role in immune responses that regulate the development of atopic diseases (e.g., asthma, allergic rhinitis, atopic dermatitis). Human TIM-1 is a functional receptor for hepatitis A virus (HAV). In humans, certain polymorphic variants of TIM-1 are strongly associated with protection against atopy, and this association occurs only in individuals who have had past infection with hepatitis A virus (HAV). Activation of T cells through TIM-1 by HAV or by its natural ligand may affect T cell differentiation and the development of Th2-driven allergic inflammatory responses.
Usage
For research use only, not for diagnostic or therapeutic use. The Tim-1-Fc fusion protein has been reported for use in immunofluorescent staining of Tim-1 ligand expressing cells.
References
McIntire, J., D. Umetsu, and R. DeKruyff. 2004. Tim-1, a novel allergy and asthma susceptibility gene. Spinger Semin Immun. 25-335-348.
Khademi, M., et al. 2004. T cell Ig- and mucin-domain-containing molecule 3 (Tim-3) and Tim-1 molecules are differentially expressed on human Th1 and Th2 cells and in cerebrospinal fluid-derived mononuclear cells in multiple sclerosis. J. Immunol. 172: 7169-7176.
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