Contents: Recombinant Mouse Tumor Necrosis Factor alpha (TNF-á)
Catalog Number: 14-8321
Sizes: 5 ug, 10 ug
Formulation: Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with 0.5% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -20°C in a non-cycling freezer (e.g., not 'frost free'). Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF-á (accession # NM_013693).
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides on SDS-PAGE.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse L929 cytotoxicity assay. The ED₅₀ is 5.0 pg/ml, corresponding to a specific activity of 2.0 x 10⁸ Units/mg.

 

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