Format: Supernatant; Reactivity: human, mouse
Articlecode: 03-57033
Product: Anti-U1 70K Protein (Human autoantigens)
Catalog #: 03-57033
Amount: 200 ml
CATEGORY: Mouse monoclonal
CLONE: H111
IMMUNOGLOBULIN CLASS: IgG2a
FORM: Cell culture supernatant
IMMUNOGEN: Purified U1 snRNPs from HeLa cells
DESCRIPTION/SPECIFICITY: The Mab reacts specifically with the U1-70K protein in a variety of species ranging between human and mouse. It precipitates U1 snRNP cpmplexes containing the U1-70K protein.
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Human, mouse
APPLICATION: Immunoblotting (Western) (U1-70K protein)
Immunoprecipitation (U1 snRNPs)
RECOMMENDED FOR POSITIVE CONTROL: HEp-2 cells
WORKING DILUTION: Immunoblotting: 1:5 to 1:10
DILUTION BUFFER: PBS containing 1% BSA and 0.09% sodium azide
STORAGE: 2-8°C for immediate use; or at -20°C (aliquot)
REFERENCES:
Manual of Biological Markers of Disease (1993) Section A (Eds. W.J. van Venrooij & R.N. Maini),
Kluwer Acad. Publ. Dordrecht
Kastner et al. (1992) J. Cell Biol. 116: 839-849
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