Format: Supernatant; Reactivity: human (most species)
Articlecode: 03-57034
Product: Anti-U1 snRNP A Protein (Human autoantigen)
Catalog #: 03-57034
Amount: 200 ml
CATEGORY: Mouse monoclonal
CLONE: H304
IMMUNOGLOBULIN CLASS: IgG1
FORM: Cell culture supernatant
DESCRIPTION/SPECIFICITY: The Mab reacts primarily with the human U1A protein in a variety of species ranging between human and Xenopus laevis. It precipitates U1snRNP complexes containing The U1A protein. It does not cross react with the U2B” protein
ANTIGEN RECOGNIZED IN SPECIES: Most species
APPLICATION: Immunoblotting (Western)
Immunoprecipitation
RECOMMENDED FOR POSITIVE CONTROL: HEp-2 cells
WORKING DILUTION: Immunoblotting: 1:5 to 1:10
Immunoprecipitation: 1:5 to 1:10
Optimal dilution should be determined by serial dilution.
DILUTION BUFFER: PBS containing 1% BSA and 0.09% sodium azide
STORAGE: 2-8°C for immediate use; or at -20°C (aliquot)
REFERENCES:
Manual of Biological Markers of Disease (1993) Section A (Eds. W.J. van Venrooij & R.N. Maini),
Kluwer Acad. Publ. Dordrecht
Reuter, R & Luhrmann R. (1986) PNAS USA 83: 8689-8693
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