Format: Supernatant; Reactivity: human (other mammals)
Articlecode: 03-57036
Product: Anti-U2 snRNP B” Protein (Human autoantigen)
Catalog #: 03-57036
Amount: 200 ml
CATEGORY: Mouse monoclonal
CLONE: 4G3
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Recombinant b-galactosidase U2B” fusion protein
FORM: Cell culture supernatant
DESCRIPTION/SPECIFICITY: The Mab reacts primarily with the U2B” protein from a variety of species ranging from human to plant. It does not react detectably with the very homologous U1A protein
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Most species including human and plant
APPLICATION: Immunoblotting (Western)
Immunofluorescence microscopy
Immunoprecipitation
RECOMMENDED FOR POSITIVE CONTROL: HEp-2 cells
WORKING DILUTION: Immunoblotting: 1:5 to 1:10
Immunofluorescence microscopy: 1:5 to 1:10
DILUTION BUFFER: PBS containing 1% BSA and 0.09% sodium azide
STORAGE: 2-8°C for immediate use; or at -20°C (aliquot)
REFERENCES:
Manual of Biological Markers of Disease (1993) Section A (Eds. W.J. van Venrooij & R.N. Maini),
Kluwer Acad. Publ. Dordrecht
Habets, WJ. Et al. (1989) J. Immunol 143: 2560-2566
Simpson et al. (1991) Nucleic Acids Research 19: 5213-5217
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