Reactivity: human; Vesicular acetylcholine Transporter
Articlecode: 03-16095
Product: Anti- Human Vesicular acetylcholine Transporter (VAChT)
Catalog #: 03-16095
Amount: 100 ml
CATEGORY: Rabbit Polyclonal
FORM: Serum containing 0.1% sodium azide
IMMUNOGEN: Synthetic peptide from the C-terminus of human VAChT, conjugated to BSA
DESCRIPTION/SPECIFICITY: The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS. Absorption with 10-100 mg immunogen per ml diluted antiserum abolishes staining.
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Human
RECOMMENDED FOR POSITIVE CONTROL: Frozen sections of human small intestine
APPLICATION: Immunohistochemistry, suitable for frozen sections.
INCUBATION TIME: Overnight at 2-8°C
WORKING DILUTION: 1:40 to 1:60 using FITC conjugates as secondary antibody
DILUTION BUFFER: 0.1 M PBS with 1% BSA and 0.1% Na-Azide
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
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